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JRA 競走馬総合研究所

トップページ > 研究室紹介 > 分子生物研究室 Molecular Biology Division

研究室紹介

分子生物研究室 Molecular Biology Division

ウイルス性疾患から日本の馬産業を守れ!

分子生物研究室イメージ

分子生物研究室では、馬のさまざまなウイルス性疾患を対象に、疫学調査、診断法の開発、予防や治療に関する研究を行っています。とりわけ、国内で流産や発熱を引き起こして問題となっている馬ヘルペスウイルス1型感染症(馬鼻肺炎)、および馬インフルエンザに関しては、競走資源の損失あるいは円滑な競馬開催の妨げとなりうることから、精力的にそれらの防あつに直結する研究に取り組んでいます。そのほか、子馬の主な下痢症の原因である馬ロタウイルス感染症や、馬コロナウイルス感染症、ゲタウイルス感染症などの研究を行っています。
また、当研究室は2021年6月より、馬インフルエンザのOIEリファレンスラボラトリーに認定されています。

パンフレットbrochure(PDF:3.2MB)

研究スタッフ  Researcher

  • 室長Manager太田 稔Minoru OHTA, DVM, PhD
  • 上席研究役Chief researcher辻村行司Koji TSUJIMURA, DVM, PhD
  • 研究役 Researcher根本 学Manabu NEMOTO, DVM, PhD
  • 研究役Researcher坂内 天Hiroshi BANNAI, DVM, PhD
  • 主査Researcher 上林義範Yoshinori KAMBAYASHI, DVM

研究課題  Research project

一般研究(2019-2021)
リバースジェネティクス法による馬インフルエンザウイルスのヘマグルチニン(HA)遺伝子を保有する人工ウイルスの作出
共同研究(2019-2021)
ウマ腸管オルガノイドの確立およびウマ下痢症ウイルス分離の試み
一般研究(2021-2023)
馬感染症の多病原体同時検出法の確立に関する研究

原著論文  Original article

2021

Bannai H,  Takahashi Y, Ohmura H, Ebisuda Y, Mukai K, Kambayashi Y, Nemoto M, Tsujimura K, Ohta M, Raidal S, Padalino B
Decreased virus-neutralizing antibodies against equine herpesvirus type 1 in nasal secretions of horses after 12-hour transportation
J Equine Vet Sci 103, 103665, 2021 [PubMed]

Bannai H, Tominari M, Kambayashi Y, Nemoto M, Tsujimura K, Ohta M
Evaluation of antibody response in horses after vaccination with an inactivated Getah virus vaccine using an accelerated immunization schedule
J Equine Vet Sci 99, 103396, 2021 [PubMed]

Nemoto M, Ohta M, Yamanaka T, Kambayashi Y, Bannai H, Tsujimura K, Yamayoshi S, Kawaoka Y, Cullinane A
Antigenic differences between equine influenza virus vaccine strains and Florida sublineage clade 1 strains isolated in Europe in 2019
Vet J 272, 105674, 2021 [PubMed]

Kambayashi Y, Bannai H, Tsujimura K, Hirama A, Ohta M, Nemoto M
Outbreak of equine coronavirus infection among riding horses in Tokyo, Japan
Comp Immunol Microbiol Infect Dis 77, 101668, 2021 [PubMed]

Nemoto M, Matsumura T
Equine rotavirus infection
J Equine Sci 32, 1-9, 2021 [PubMed]

2020

Sato W, Sukmawinata E, Uemura R, Kanda T, Kusano K, Kambayashi Y, Sato T, Ishikawa Y, Toya R, Sueyoshi M
Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan
J Equine Sci 31, 85-91, 2020 [PubMed]

Ohta M, Bannai H, Nemoto M, Kambayashi Y, Tamura N, Tsujimura K
Antibody responses against equine influenza virus induced by concurrent and by consecutive use of an inactivated equine influenza virus vaccine and a modified live equine herpesvirus type 1 vaccine in Thoroughbred racehorses
J Equine Vet Sci 94, 103221, 2020 [PubMed]

Nemoto M, Okita N, Kitahata M, Bannai H, Tsujimura K, Kinoshita Y, Kambayashi Y, Cullinane A, Yamanaka T, Ohta M
Evaluation of cobas Influenza A/B & RSV test for diagnosis of equine influenza
J Equine Vet Sci 94, 103249, 2020 [PubMed]

Sukmawinata E, Sato W, Uemura R, Kanda T, Kusano K, Kambayashi Y, Sato T, Ishikawa Y, Toya R, Sueyoshi M
Antimicrobial-resistant Enterococcus faecium and Enterococcus faecalis isolated from healthy Thoroughbred racehorses in Japan
J Equine Vet Sci 94, 103232, 2020 [PubMed]

Bannai H, Nemoto M, Tsujimura K, Ohta M
Establishment of an enzyme-linked immunosorbent assay for Getah virus infection in horses using a 20-mer synthetic peptide for the E2 glycoprotein as an antigen
Arch Virol 165, 377-385, 2020 [PubMed]

Cullinane A, Gahan J, Walsh C, Nemoto M, Entenfellner J, Olguin-Perglione C, Garvey M, Huang Fu TQ, Venner M, Yamanaka T, Barrandeguy M, Fernandez CJ
Evaluation of current equine influenza vaccination protocols prior to shipment, guided by OIE standards
Vaccines 8, 107, 2020 [PubMed]

Nemoto M, Niwa H, Kida H, Higuchi T, Orita Y, Sato S, Bannai H, Tsujimura K, Ohta M
Isolation and characterization of a rare group A rotavirus G13P[18] strain from a diarrhoeic foal in Japan
J Gen Virol 101, 800-805, 2020 [PubMed]

2019

El-Hage CM, Bannai H, Wiethoelter AK, Firestone SM, Heislers CM, Allen JL, Waller AS, Gilkerson JR
Serological responses of Australian horses using a commercial duplex indirect ELISA following vaccination against strangles
Aus Vet J 97, 220-224, 2019 [PubMed]

Nemoto M, Niwa H, Murakami S, Miki R, Higuchi T, Bannai H, Tsujimura K, Kokado H
Molecular analyses of G3A/G3B and G14 equine group A rotaviruses detected between 2012 and 2018 in Japan
J Gen Virol 100, 913-931, 2019 [PubMed]

Takehana K, Kinjyo T, Nemoto M, Matsuno K
Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)
J Vet Med Sci 81, 504-507, 2019 [PubMed]

Tsujimura K, Bannai H, Nemoto M, Kokado H
Loop-mediated isothermal amplification-fluorescent loop primer assay for the genotyping of a single nucleotide polymorphism at position 2254 in the viral DNA polymerase gene of equid alphaherpesvirus 1
J Vet Diagn Invest 31, 640-644, 2019 [PubMed]

Nemoto M, Yamayoshi S, Bannai H, Tsujimura K, Kokado H, Kawaoka Y, Yamanaka T
A single amino acid change in hemagglutinin reduces the cross-reactivity of antiserum against an equine influenza vaccine strain
Arch Virol 164, 2355-2358, 2019 [PubMed]

Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kokado H
Development of an enzyme-linked immunosorbent assay for Getah virus infection in horses using recombinant E2 protein as an antigen
J Virol Methods 271, 113681, 2019 [PubMed]

Bannai H, Tsujimura K, Nemoto M, Ohta M, Yamanaka T, Kokado H, Matsumura T
Epizootiological investigation of equine herpesvirus type 1 infection among Japanese racehorses before and after the replacement of an inactivated vaccine with a modified live vaccine
BMC Vet Res 15, 280, 2019 [PubMed]

Nemoto M, Schofield W, Cullinane A
The first detection  of equine coronavirus in adult horses and foals in Ireland
Viruses 11, 946, 2019 [PubMed]

Nemoto M, Tamura N, Bannai H, Tsujimura K, Kokado H, Ohta M, Yamanaka T
Mutated influenza A virus exhibiting reduced susceptibility to baloxavir marboxil from an experimentally infected horse
J Gen Virol 100, 1471-1477, 2019 [PubMed]

Ogata M, Yamanaka T, Koizumi A, Sakamoto M, Aita R, Endo H, Yachi T, Yamauchi N, Otsubo T, Ikeda K, Kato T, Park EY, Kono H, Nemoto M, Hidari KIPJ
Application of novel sialoglyco particulates enhances the detection sensitivity of the equine influenza virus by real-time reverse transcriptase polymerase chain reaction
ACS Appl Bio Mater 2, 1255-1261, 2019

2018

Nemoto M, Yamanaka T, Bannai H, Tsujimura K, Kokado H
Complete genomic sequences of H3N8 equine influenza virus strains used as vaccine strains in Japan
Genome Announc 6, e00172-18, 2018 [PubMed]

Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Matsumura T, Kokado H, Gildea S, Cullinane A
Neutralization antibody response to booster/priming immunization with new equine influenza vaccine in Japan
J Vet Med Sci 80, 382-386, 2018 [PubMed]

Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kokado H, Kondo T, Matsumura T
Comparison of protective efficacies between intranasal and intramuscular vaccination of horses with a modified live equine herpesvirus type-1 vaccine
Vet Microbiol 222, 18-24, 2018 [PubMed]

Nemoto M, Yamanaka T, Bannai H, Tsujimura K, Ueno T, Mekata H, Yoshida A, Koyama A, Kokado H
Comparison of two agar gel immunodiffusion protocols for diagnosing equine infectious anemia
J Vet Med Sci 80, 1245-1247, 2018 [PubMed]

Nemoto M, Inagaki M, Tamura N, Bannai H, Tsujimura K, Yamanaka T, Kokado H
Evaluation of inactivated vaccines against equine group A rotaviruses by use of a suckling mouse model
Vaccine 36, 5551-5555, 2018 [PubMed]

Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kokado H, Kondo T
Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
J Equine Sci 29, 111-115, 2018 [PubMed]

Kuwata R, Shimoda H, Phichitraslip T, Prasertsincharoen N, Noguchi K, Yonemitsu K, Minami S, Supriyono, Tran NTB, Takano A, Suzuki K, Nemoto M, Bannai H, Yokoyama M, Takeda T, Jittapalapong S, Rerkamnuaychoke W, Maeda K
Getah virus epizootic among wild boars in Japan around 2012
Arch Virol 163, 2817-2821, 2018 [PubMed]

2017

Bannai H, Nemoto M, Niwa H, Murakami S, Tsujimura K, Yamanaka T, Kondo T
Geospatial and temporal association of Getah virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015
BMC Vet Res 13, 187, 2017 [PubMed]

Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Fu TQH, Fernandez CJ, Gildea S, Cullinane A
Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system
J Vet Med Sci 79, 1061-1063, 2017 [PubMed]

Nemoto M, Bannai H, Ochi A, Niwa H, Murakami S, Tsujimura K, Yamanaka T, Kokado H, Kondo T
Complete genome sequences of Getah virus strains isolated from horses in 2016 in Japan
Genome Announc 5, e00750-17, 2017 [PubMed]

Nemoto M, Ryan E, Lyons P, Cullinane A
Molecular characterisation of equine group A rotaviruses in Ireland (2011-2015)
Vet J 226, 12-14, 2017 [PubMed]

Nemoto M, Kanno T, Bannai H, Tsujimura K, Yamanaka T, Kokado H
Antibody response to equine coronavirus in horses inoculated with a bovine coronavirus vaccine
J Vet Med Sci 79, 1889-1891, 2017 [PubMed]

Ogata M, Koizumi A, Otsubo T, Ikeda K, Sakamoto M, Aita R, Kato T, Park EY, Yamanaka T, Hidari KIPJ
Chemoenzymatic synthesis and characterization of N-glycolylneuraminic acid-carrying sialoglycopolypeptides as effective inhibitors against equine influenza virus hemagglutination
Biosci Biotechnol Biochem 81, 1520-1528, 2017 [PubMed]

2016

Bannai H, Ochi A, Nemoto M, Tsujimura K, Yamanaka T, Kondo T
A 2015 outbreak of Getah virus infection occurring among Japanese racehorses sequentially to an outbreak in 2014 at the same site
BMC Vet Res 12, 98, 2016 [PubMed]

Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Maeda K, Kondo T
Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen
J Vet Med Sci 78, 309-311, 2016 [PubMed]

Nemoto M, Bannai H, Tsujimura K, Yamanaka T, Kondo T
Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan
Arch Virol 161, 1691-1695, 2016 [PubMed]

Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Gildea S, Cullinane A
Evaluation of twenty-two rapid antigen detection tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype
Influenza Other Respir Viruses 10, 127-133, 2016 [PubMed]

Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Gildea S, Cullinane A
Assessment of antigenic differences of equine influenza virus strains by challenge study in horses
Influenza Other Respir Viruses 10, 536-539, 2016 [PubMed]

樋口 徹・井上 哲・佐藤正人・秦 賢司・辻村行司
外科的切除と凍結した組織片移植により治療した馬サルコイドの1症例
北獣会誌 60, 423-426, 2016

2015

Andoh K, Hattori S, Mahmoud HY, Takasugi M, Shimoda H, Bannai H, Tsujimura K, Matsumura T, Kondo T, Kirisawa R, Mochizuki M, Maeda K
The haemagglutination activity of equine herpesvirus type 1 glycoprotein C
Virus Res 195, 172-176, 2015 [PubMed]

Yamanaka T, Cullinane A, Gildea S, Bannai H, Nemoto M, Tsujimura K, Kondo T, Matsumura T
The potential impact of a single amino-acid substitution on the efficacy of equine influenza vaccines
Equine Vet J 47, 456-462, 2015 [PubMed]

Nemoto M, Morita Y, Niwa H, Bannai H, Tsujimura K, Kondo T, Matsumura T
Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplification
J Virol Method 215-216, 13-16, 2015 [PubMed]

Nemoto M, Nagai M, Tsunemitsu H, Omatsu T, Furuya T, Shirai J, Kondo T, Fujii Y, Todaka R, Katayama K, Mizutani T
Whole-genome sequence analysis of G3 and G14 equine group A rotaviruses isolated in the late 1990s and 2009-2010
Arch Virol 160, 1171-1179, 2015 [PubMed]

Nemoto M, Bannai H, Tsujimura K, Kobayashi M, Kikuchi T, Yamanaka T, Kondo T
Getah virus infection among racehorses, Japan, 2014
Emerg Infect Dis 21, 883-885, 2015 [PubMed]

Bannai H, Nemoto M, Ochi A, Kikuchi T, Kobayashi M, Tsujimura K, Yamanaka T, Kondo T
Epizootiological investigation of getah virus infection among racehorses in Japan in 2014
J Clin Microbiol 53, 2286-2291, 2015 [PubMed]

Tsujimura K, Murase H, Bannai H, Nemoto M, Yamanaka T, Kondo T
Efficacy of five commercial disinfectants and one anionic surfactant against equine herpesvirus type 1
J Vet Med Sci 77, 1545-1548, 2015 [PubMed]

Nemoto M, Oue Y, Murakami S, Kanno T, Bannai H, Tsujimura K, Yamanaka T, Kondo T
Complete genome analysis of equine coronavirus isolated in Japan
Arch Virol 160, 2903-2906, 2015 [PubMed]

Nemoto M, Oue Y, Higuchi T, Kinoshita Y, Bannai H, Tsujimura K, Yamanaka T, Kondo T
Low prevalence of equine coronavirus in foals in the largest thoroughbred horse breeding region of Japan, 2012-2014
Acta Vet Scand 57, 53, 2015 [PubMed]

学会発表  Conference presentation

2020

坂内 天・上林義範・根本 学・辻村行司・太田 稔
競走馬群におけるウマヘルペスウイルス1型ワクチンの接種対象拡大の効果
第163回日本獣医学会学術集会 2020.9

上林義範・坂内 天・根本 学・辻村行司・平間あき子・太田 稔
サラブレッドを含む乗用馬群における馬コロナウイルス感染症の流行
第163回日本獣医学会学術集会 2020.9

坂内 天・上林義範・根本 学・辻村行司・太田 稔
JRA競走馬および育成馬におけるウマコロナウイルスの浸潤状況
日本ウマ科学会第33回学術集会 2020.11

2019

Bannai H
Outbreaks of Getah virus infection among racehorses in Japan: Lessons and research trends
Conference on Cooperation and Collaboration on Prevention and Control of Animal Diseases 2019.5

根本 学・坂内 天・辻村行司・古角 博・山中隆史
ゾフルーザ(バロキサビル マルボキシル)の馬インフルエンザに対する有効性
第33回インフルエンザ研究者交流の会シンポジウム 2019.6

Nemoto M
Coronavirus
Havemeyer Workshop 2019, Acute Equine Colitis 2019.7

2018

根本 学・稲垣瑞穂・田村周久・坂内 天・辻村行司・古角 博
マウスモデルによるウマロタウイルスワクチンの評価
第161回日本獣医学会学術集会 2018.9

Tsujimura K, Bannai H, Nemoto M, Kokado H, Matsumura T
Simple and rapid detection and differentiation of equine herpesvirus type 1 strains with A2254 and G2254 polymorphisms by LAMP assay using a fluorescent loop primer
4th Havemeyer Foundation Workshop on Equine Herpesviruses 2018.10

Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kokado H, Kondo T, Matsumura T
Systemic and mucosal immune responses in horses infected with equine herpesvirus type-1 and the protective effect after subsequent challenge
第66回日本ウイルス学会学術集会 2018.10

2017

Bannai H
Investigating factors causing outbreaks of Getah virus infection among racehorses in Japan
2017 Australian and New Zealand College of Veterinary Scientists (ANZCVS), College Science Week 2017.7

2016

山中隆史
馬の監視伝染病 -ウイルス性疾患-
平成27年度日本獣医師会獣医学術学会年次大会 2016.2

Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kondo T
Protective effect of intranasal vaccination of horses with a modified live equine herpesvirus type 1 vaccine
10th International Equine Infectious Disease Conference 2016.4

Nemoto M, Bannai H, Tsujimura K, Yamanaka T, Kondo T
Genetic and antigenic analysis of Getah virus isolated in 1978 and 2014 in Japan
10th International Equine Infectious Disease Conference 2016.4

Tsujimura K, Yamamoto A, Miyazawa M, Bannai H, Nemoto M, Yamanaka T, Ozawa M, Kato K, Kondo T
Application of ORF68-based grouping to an epidemiological survey of epidemic abortions caused by equine herpesvirus type 1
10th International Equine Infectious Disease Conference 2016.4

Yamanaka T, Bannai H, Nemoto M, Tsujimura K, Kondo T, Matsumura T, Gildea S, Cullinane A
Assessment of antigenic difference of equine influenza virus by challenge study in horses
10th International Equine Infectious Disease Conference 2016.4

山中隆史・坂内 天・根本 学・辻村行司・近藤高志・松村富夫・Gildea S・Cullinane A
馬体への攻撃試験による馬インフルエンザウイルスの抗原性の解析
第30回インフルエンザ研究者交流の会シンポジウム 2016.6

Bannai H
Epizootic Getah virus infection among racehorses in Japan
12th Annual Meeting of the Australian Association of Veterinary Laboratory Diagnosticians 2016.11

2015

Yamanaka T, Nemoto M, Bannai H, Kondo T, Matsumura T,  Gildea S, Cullinane A
The evaluation of twenty rapid antigen detection (RAD) tests in the diagnosis of equine influenza
3rd International Symposium on Neglected Influenza Viruses 2015.4

Bannai H, Nemoto M, Ochi A, Kikuchi T, Kobayashi M, Tsujimura K, Yamanaka T, Kondo T
Investigation of factors causing an outbreak of getah virus infection among racehorses in Japan 2014
第63回日本ウイルス学会学術集会 2015.11

坂内 天・根本 学・辻村行司・山中隆史・近藤高志
JRA施設における2014年のゲタウイルス感染症の発生
第22回トガ・フラビ・ペスチウイルス研究会 2015.11